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本文报道制备固定化苯丙氨酸解氨酶(PAL)的初步结果。采用包埋和共价结合两种方法将PAL固定化。结果表明前者的偶联率高于后者。冷冻干燥,4°下贮存6个月后,活性存留分别为89％和76％,说明固定化后稳定性良好。包埋法采用不同T/C比(T为凝胶总浓度;C为交联百分比)的聚丙烯酰胺凝胶(PAG)包埋,结果证明按T/C比为3:30时固定化的酶活性明显高于T/C比为8:19者,二者的相对活性分别为58％和6％。用戊二醛将PAL固定于Bio—Gel P—300上的酶活性高于用碘化—1—[环已基—3—(3—二甲氨丙基)]碳二亚胺(CDC)固定化的酶,相对活性分别为63％和18％。
This article reports the preliminary results of preparing immobilized phenylalanine ammonia lyase (PAL). PAL was immobilized using both methods of entrapment and covalent attachment. The results show that the former coupling rate higher than the latter. After freeze-drying, the viability was 89% and 76%, respectively, after 6 months storage at 4 ° C, indicating good stability after immobilization. Embedding method using different T / C ratio (T is the total gel concentration; C is cross-linked percentage) polyacrylamide gel (PAG) embedding, the results show that at a T / C ratio of 3:30 immobilized Enzyme activity was significantly higher than the T / C ratio of 8:19, the relative activity of the two were 58% and 6%. The enzyme activity of PAL immobilized on Bio-Gel P-300 with glutaraldehyde is higher than that of PAL with iodinated 1- [cyclohexyl-3- (3-dimethylaminopropyl)] carbodiimide (CDC) Immobilized enzymes, relative activities were 63% and 18%, respectively.