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目的 探讨老年人群 Rb基因 2 0内含子的 VNTR多态性分布 ,并与青年群体比较是否发生改变 ,以此研究老年人的衰老机制 .方法 对 5 6例陕西青年、6 8例老年人群用PCR- VNTR方法进行 Rb基因 2 0内含子多态性的检测 .结果 在青年群体发现 :等位基因片段 10个 ,片段大小为330～ 2 6 0 bp,重复片段大小为 5 bp,频率分布在 0 .0 0 9～0 .2 95 .该位点杂合率为 :0 .70 ,PIC(信息量 ) :0 .86 .老年人群多态片段增多至 18个等位基因片段 ,范围在 2 40～ 390 bp,青年人群及老年人群的 Rb基因 VNTR位点的多态分布差异有显著性 (P<0 .0 0 1) ,同时发现 VNTR重复数量 :老年人群 >青年人群 .结论 Rb基因 VNTR重复数量的增多可能是老年人群衰老的机制之一
Objective To investigate the distribution of VNTR polymorphism in 20 intron of Rb gene in the elderly population and to compare with the youth population whether there is any change in the aging population.Methods 566 Shaanxi youth and 68 elderly PCR-VNTR method was used to detect the polymorphism of 20 Rb genes.Results In the youth population, there were 10 alleles, 330-260 bp in size, 5 bp in size and 5 bp in frequency At 0 0 0 0 ~ 0 2 95. The heterozygosity at this locus was 0.70 and the PIC (informative) was 0.86. The number of polymorphic fragments in the elderly population increased to 18 alleles ranging from 2 40 ~ 390 bp, the difference of the polymorphism of VNTR locus in Rb gene between young and old population was significant (P <0.01), and the number of VNTR repeat was found in elderly population> young population.Conclusion Rb gene The increased number of VNTR repeats may be one of the mechanisms of aging in the elderly