论文部分内容阅读
分别采用紫外分光光度计法、滴定法、电化学法研究了莲原花青素对金属离子的配合物配合率值。结果表明,3种方法测量配合率值时各有利弊,并分别找出3种方法适用的莲原花青素与金属离子配合率测定的不同浓度范围。其中极谱法适用于(1~10)mg/kg莲原花青素,紫外分光光度计法适用于(10~100)mg/kg莲原花青素,滴定法适用于较高浓度莲原花青素(1000 mg/kg)与金属离子配合率的测定。在实验过程中还可以得出p H是影响配合物形成的重要因素。Fe~(3+)、Fe~(2+)、Cu~(2+)、Pb~(2+)离子在酸性条件下即可与莲原花青素配合,而Zn~(2+)、Mn~(2+)、Ca~(2+)在溶液p H更高时才能与莲原花青素配合。
UV spectrophotometer method, titration method and electrochemical method were used to study the complexation ratio of lotus procyanidins to metal ions. The results showed that each of the three methods had its own advantages and disadvantages when measuring the compounding rate, and different concentration ranges for the determination of the blending ratio of lotus procyanidins and metal ions by three methods were respectively found out. The polarographic method is suitable for (1 ~ 10) mg / kg lotuanthocyanidins and ultraviolet spectrophotometer is suitable for (10 ~ 100) mg / kg lotuanthocyanin. The titrimetric method is suitable for the high concentration lotus procyanidins (1000 mg / kg) Determination of metal ion coordination rate. It can also be concluded during the experiment that p H is an important factor affecting the formation of complexes. Fe ~ (3 +), Fe ~ (2+), Cu ~ (2 +) and Pb ~ (2+) ions could coordinate with lotus proanthocyanidins under acidic conditions, but Zn 2+, Mn ~ 2 +), Ca ~ (2+) can be complexed with lotus proanthocyanidins when the solution pH is higher.