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目的:研究结核性胸膜炎与恶性胸水患者胸腔积液单个核细胞经结核分枝杆菌特异性抗原肽刺激后IL-22的表达特点,探索IL-22对结核性胸膜炎和恶性胸水的鉴别诊断价值。方法:采用eBioscience IL-22和IFN-γ试剂盒,利用流式细胞术微球阵列法检测52例结核性胸膜炎和35例恶性胸水患者胸水上清、PFMCs经结核分枝杆菌特异性抗原肽刺激培养后上清中IL-22和IFN-γ的浓度,并作统计学分析。结果:结核性胸膜炎组IL-22在刺激后上清中表达水平显著升高,且显著高于恶性胸水组(P<0.05)。结核性胸膜炎组IL-22与IFN-γ在刺激后上清中表达水平显著相关(r=0.3485,P=0.0320)。结论:结核分枝杆菌抗原特异反应性IL-22可作为鉴别诊断胸腔积液病因的新指标。
Objective: To investigate the expression of IL-22 in pleural effusion mononuclear cells of patients with tuberculous pleural effusion and malignant pleural effusion stimulated by Mycobacterium tuberculosis specific antigen peptide and to explore the differential diagnosis value of IL-22 on tuberculous pleurisy and malignant pleural effusion. Methods: 52 patients with tuberculous pleurisy and 35 patients with malignant pleural effusion were detected by flow cytometry microspheres array method using eBioscience IL-22 and IFN-γ kit. PFMCs were stimulated with Mycobacterium tuberculosis specific antigen peptide The concentrations of IL-22 and IFN-γ in the supernatant after culture were analyzed and statistically analyzed. Results: The expression of IL-22 in tuberculous pleurisy group was significantly higher than that in malignant pleural effusion group (P <0.05). The levels of IL-22 and IFN-γ in tuberculous pleurisy group were significantly correlated with the supernatant level after stimulation (r = 0.3485, P = 0.0320). Conclusion: Mycobacterium tuberculosis antigen-specific reactive IL-22 can be used as a new differential diagnosis of pleural effusion etiology.