论文部分内容阅读
目的探索通过PET/CT显像将中药有效成分在体内的代谢过程变成可视的图像,并通过图像对其进行定量分析的可行性。方法以延胡索的有效成分左旋千金藤啶碱(L-SPD)作为研究对象。使用正电子核素11碳(11C)标记L-SPD,在热室内化学合成11C-L-SPD。SD大鼠戊巴比妥钠麻醉后固定于木板上,尾iv 37 MBq 11C-L-SPD。分别于注射后5、15、30、45、60、90 min行PET/CT显像。使用工作站获得脑、心脏、肺脏、肝脏、肾脏、肠腔、膀胱的放射性容积分布比值(distribution volume ratios,DVR)。结果 PET/CT显像显示在5 min时11C-L-SPD在肝脏、肾脏分布最多,心脏、肺、脑可见放射性分布。肝、肾是11C-L-SPD的主要代谢及排泄器官,肝、肾、肠腔、膀胱在5 min DVR分别为(1.37±0.42)%、(1.10±0.19)%、(0.89±0.18)%、(0.97±0.11)%,90 min分别为(0.65±0.11)%、(0.54±0.05)%、(5.49±1.44)%、(9.86±1.88)%。结论 PET/CT显像可以直观、动态地观察L-SPD在活体内的分布及代谢特点,有望用于其他中药有效成分的活体显像研究。
Objective To explore the feasibility of using PET / CT imaging to turn the metabolic process of traditional Chinese medicine in vivo into a visual image and quantitatively analyze it through images. Methods L-SPD, the active ingredient of corydalis, was used as the research object. The 11C-L-SPD was chemically synthesized in a hot chamber using a positron-11 carbon (11C) labeled L-SPD. SD rats were anesthetized with pentobarbital sodium and fixed on a wooden plate with a tail of 37 MBq 11C-L-SPD. PET / CT scans were performed at 5, 15, 30, 45, 60 and 90 minutes after injection. Workstations were used to obtain the distribution volume ratios (DVRs) of the brain, heart, lung, liver, kidney, intestine and bladder. Results PET / CT imaging showed that 11C-L-SPD was the most abundant in the liver and kidneys at 5 min, and radioactively distributed in heart, lung and brain. The liver and kidney were the major metabolites and excretion organs of 11C-L-SPD. The DVR of the liver, kidney, intestine and bladder in 5 min was (1.37 ± 0.42)%, (1.10 ± 0.19)% and (0.89 ± 0.18) , (0.97 ± 0.11)% and (0.65 ± 0.11)%, (0.54 ± 0.05)%, (5.49 ± 1.44)% and (9.86 ± 1.88)% respectively at 90 min. Conclusion PET / CT imaging can directly and dynamically observe the distribution and metabolism characteristics of L-SPD in vivo and is expected to be used for the in vivo imaging studies of the active ingredients of other traditional Chinese medicines.