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观察椎管内注射感觉神经元特异性受体选择性激动剂牛肾上腺髓质8-22肽(bovine adrenal me-dulla 8-22,BAM8-22)在福尔马林引起的持续性痛中对吗啡耐受的影响.结果表明,吗啡(20μg)在正常大鼠中能显著抑制福尔马林引起的痛行为,多次应用吗啡导致耐受后,其抑制痛行为的能力大大减弱(P<0.001);吗啡耐受后,BAM8-22(0.1 nmol)能翻转吗啡的耐受效应,福尔马林引起第2期抬足/舔爪持续时间和抖动次数分别减少了39.4%(P<0.001)和25.3%(P<0.05).每天椎管内联合应用BAM8-22(0.1 nmol)和吗啡(20μg)不影响吗啡耐受的形成,但隔天给予BAM8-22则能有效延缓吗啡耐受的形成,福尔马林引起第2期缩足舔爪和抖动分别为14.7 min和234.5次,与吗啡耐受鼠相比,分别减少了41.0%(P<0.001)和24.8%(P<0.05).然而,BAM8-22并没有完全翻转或抑制吗啡的耐受(P<0.001).此外,连续6 d给予BAM8-22后会降低吗啡在福尔马林持续痛中的抗伤害作用.证明在福尔马林引起的持续性痛中,SNSR受体参与了调制阿片受体的敏感性.同时,提示在持续性痛中,通过BAM8-22延缓或翻转吗啡耐受是利用阿片受体治疗持续性痛的有效方法.
To observe the effect of injecting Bovine adrenal me-dulla 8-22 (BAM8-22), a selective agonist of sensory neuron-specific neurons in spinal canal, on persistent pain induced by formalin Morphine tolerance.The results showed that morphine (20μg) could significantly inhibit the pain induced by formalin in normal rats, and its ability to inhibit the pain behavior was greatly weakened after multiple morphine-induced tolerance (P < 0.001). After morphine tolerance, BAM8-22 (0.1 nmol) reversed the tolerance effect of morphine, and the formalin-induced decrease in duration and jitter of leg raising / licking paw2 was 39.4% (P <0.001 ) And 25.3% (P <0.05) .BAM8-22 (0.1 nmol) and morphine (20 μg) combined with spinal intrathecal administration did not affect the morphine tolerance, but BAM8-22 could delay the morphine tolerance (P <0.001) and 24.8% (P <0.05), respectively, in morphine-tolerant mice compared with morphine-tolerant mice at 14.7 min and 234.5 times However, BAM8-22 did not overturn or suppress morphine tolerance (P <0.001). In addition, administration of BAM8-22 for 6 consecutive days reduced the morphine-induced antinociception in formalin sustained pain It has been demonstrated that SNSR receptors are involved in the modulation of opioid receptor sensitivity in persistent pain induced by formalin.Meanwhile, it is suggested that in sustained pain, morphine tolerance is delayed or reversed by BAM8-22 using morphine Receptors for effective treatment of persistent pain.