目的　在腹腔镜胆囊切除术 (LC)中正确判断总胆管、胆囊管的解剖部位 ,减少手术误伤。　方法　将同期、同样体重的LC患者随机分成两组。显影组 ( 5 89例 )。术中采用亚甲蓝注射液 ,行胆囊穿刺造影 ,使胆囊、胆囊管、总胆管颜色显蓝。对照组 ( 5 5 3例 ) :常规腹腔镜胆囊切除术 ,未行胆囊造影术。　结果　显影组手术时间 [( 36± 9)min]、并发症发生率 ( 0 )及中转手术率 ( 1 87% )与对照组比较差异均有极显著意义 (P <0 0 1)。　结论　在LC术中行亚甲蓝胆囊造影术是一种较直观的显影技术 ,可帮助术者在LC术中正确判断Calot三角解剖结构 ,明显缩短手术时间 ,提高手术成功率 Objective To correctly determine the anatomy of the common bile duct and cystic duct during laparoscopic cholecystectomy (LC) and to reduce the risk of surgical injury. Methods The same period, the same weight of LC patients were randomly divided into two groups. Development group (5 89 cases). Intraoperative methylene blue injection, gallbladder puncture angiography, gallbladder, cystic duct, the common bile duct color blue. Control group (533 cases): conventional laparoscopic cholecystectomy, no cholecystectomy. Results The operative time [(36 ± 9) min], the incidence of complications (0) and the rate of retransmission surgery (1 87%) in the developing group were significantly different from those in the control group (P <0.01). Conclusions Methylene blue cholecystectomy is a relatively straightforward developing technique in LC, which can help the surgeon to correctly judge the anatomy of Calot triangle in LC and shorten the operation time and improve the success rate of operation