论文部分内容阅读
目的克隆细粒棘球蚴egG1Y162基因序列并进行蛋白序列对比分析。方法从细粒棘球蚴原头蚴提取mRNA,mRNA反转录为cDNA。设计特异引物,以cDNA为模板扩增egG1Y162基因,构建PUCm-T/egG1Y162重组质粒,经PCR、酶切及测序鉴定后进行序列分析。结果egG1Y162 cDNA为459bp,编码153个氨基酸;同源性比较表明,egG1Y162 cDNA与emY162同源性为95%,与eg95的同源性为30.61%。结论成功克隆egG1Y162抗原基因,egG1Y162蛋白质氨基酸序列与emY162有很高的相似性,但同其他抗原蛋白质序列存在明显差异,所以egG1Y162是一种新的抗原基因。
Objective To clone the sequence of egG1Y162 gene of Echinococcus granulosus and to compare the protein sequences. Methods The mRNA and protein of protoscoleces of Echinococcus granulosus were reverse transcribed into cDNA. Specific primers were designed and the egG1Y162 gene was amplified by cDNA. The recombinant plasmid pUCm-T / egG1Y162 was constructed and sequenced after PCR, restriction enzyme digestion and sequencing. The results showed that the egG1Y162 cDNA was 459 bp encoding a protein of 153 amino acids. The homology comparison showed that the homology of egG1Y162 cDNA with emY162 was 95% and that with eg95 was 30.61%. Conclusion The egG1Y162 antigen gene was cloned successfully. The amino acid sequence of egG1Y162 protein has a high similarity with that of emY162. However, egG1Y162 is a novel antigen gene.