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[目的]提高向日葵遗传图谱的密度和实用性。[方法]以123个来源于PAC-2和RHA-266杂交的F8代重组自交系(RILs)群体为材料,利用简单序列重复(Simple sequence repeat,SSR)标记,采用MAPMARKER软件对向日葵遗传图谱进行标注。从300对SSR引物中筛选出51对有多态性的引物对(RILs)群体进行扫描。[结果]有19对引物无多态性或条带不清晰,32对引物表现多态性;共检测到35个多态性位点,分布在图谱的15条连锁群上。标记后的图谱总长度为2914.5cM,比原来的图谱增长7.5cM。标记间平均距离由9.0cM缩短为8.1cM。[结论]为进一步的向日葵遗传图谱整合和分子标记辅助选择提供参考。
[Objective] To improve the density and practicability of the genetic map of sunflower. [Method] With 123 simple inbreeding (RILs) population of F8 hybrids derived from PAC-2 and RHA-266 as materials, simple sequence repeat (SSR) markers and MAPMARKER software were used to analyze the genetic map of sunflower Mark it. Fifty-three pairs of SSR primers were screened for 51 pairs of polymorphic primer pairs (RILs). [Result] Nineteen pairs of primers had no polymorphism or unclear bands, and 32 pairs of primers showed polymorphism. A total of 35 polymorphic loci were detected in 15 linkage groups of the map. The total length of the labeled map is 2914.5cM, which is 7.5cM more than the original map. The average distance between markers was shortened from 9.0cM to 8.1cM. [Conclusion] This provided reference for further genetic map integration and molecular marker assisted selection of sunflower.