AIM: To investigate the effect of shenfu injection on gastrointestinal microcirculation after myocardial ischemic-reperfusion (IR) injury in rabbits and probe into the mechanism. METHODS: Forty healthy flap-eared white rabbits were randomly divided into 4 groups: IR injury control group (groupⅠ), shenfu injection 5 mL/kg per h group (groupⅡ), shenfu injection 10 ml/kg per h group (groupⅢ) and shenfu injection 20 mL/kg per h group (groupⅣ). The four groups were treated with Lactated Ringer’ s solution, shenfu injection 5, 10, and 20 mL/ kg per h were infused intravenously 30 min before experiment respectively. The values of hemodynamics [mean arterial pressure (MAP), heart rate (HR), gastric intramucosal partial pressure of carbon dioxide (PCO2), blood gas analysis and pH] were measured and compared with those before myocardial ischemia, 60 min after myocardial ischemia and 60, 90, and 180 min after reperfusion. RESULTS: The MAP, HR and gastric intramucosal pH were (70.50±4.50) kPa, (165±14) beats per min, 7.032±0.024 in group I 60 min after myocardial ischemia, which were significantly decreased compared with those before myocardial ischemia (88.50±9.75 kPa, 217±18 beats per min, 7.112±0.035, P < 0.05). The MAP, HR and gastric intramucosal pH were significantly decreased in group I 60, 90, and 180 min after reperfusion (61.50±5.25 kPa, 133±31 beats per min, 6.997±0.025) compared with those before reperfusion respectively (P < 0.05), whereas the values were insignificantly different in groupsⅡ,ⅢorⅣafter reperfusion, compared with those before reperfusion, and there were no significant differences between groupsⅡ,Ⅲ, andⅣafter reperfusion. CONCLUSION: Pre-infusion of shenfu injection has a protective effect on gastrointestinal microcirculation after myocardial IR injury in rabbits, in a dose independent manner. AIM: To investigate the effect of shenfu injection on gastrointestinal microcirculation after myocardial ischemic-reperfusion (IR) injury in rabbits and probe into the mechanism. METHODS: Forty healthy flap-eared white rabbits were randomly divided into 4 groups: IR injury control group ( groupI), shenfu injection 5 mL/kg per h group (groupII), shenfu injection 10 ml/kg per h group (groupIII) and shenfu injection 20 mL/kg per h group (groupIV). The four groups were treated with Lactated Ringer ‘s solution, shenfu injection 5, 10, and 20 mL/ kg per h were in infused intravenously 30 min before respectively respectively. The values ​​of hemodynamics [mean arterial pressure (MAP), heart rate (HR), gastric intramucosal partial pressure of carbon After treatment of reperfusion of reperfusion of mitochondria (PCO2), blood gas analysis and pH] were measured and compared with those before myocardial ischemia, 60 min after myocardial ischemia and 60, 90, and 180 min after reperfusion. RESULTS: The MAP, HR and gastric intramucosal pH were (70.50 ±4.50) kPa, (165±14) beats per min, 7.032±0.024 in group I 60 min after myocardial ischemia, which was significantly decreased compared with those before ischemia (88.50±9.75 kPa, 217±18 beats per min, 7.112±0.035, P < 0.05). The MAP, HR and gastric intramucosal pH were significantly decreased in group I 60, 90, and 180 min after reperfusion (61.50±5.25 kPa, 133±31 beats per min, 6.997±0.025) compared with those before impact Respectively (P < 0.05), while the values ​​were insignificantly different in groupsII,IIIorIVafter reperfusion, compared with those before reperfusion, and there were no significant differences between groupsII,III, andIVafter reperfusion. CONCLUSION: Pre-infusion of shenfu injection has a protective Effect on gastrointestinal microcirculation after myocardial IR injury in rabbits, in a dose independent manner.