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制备了一种壳聚糖包覆的磁性纳米粒子,并对其进行了表征和蛋白吸附特性研究.首先通过共沉淀法制备了表面羧基功能化的磁性纳米粒子(MNP-COOH),然后通过静电相互作用将壳聚糖自组装在MNPCOOH粒子表面,在甲醛保护氨基的情况下,利用环氧氯丙烷交联粒子表面壳聚糖上的羟基,交联完成后脱去氨基保护剂,制得表面富含氨基的壳聚糖包覆磁性纳米粒子(MNP-CS).分别使用扫描电子显微镜(SEM)、X射线衍射仪(XRD)、傅里叶变换红外光谱仪(FTIR)、热重分析仪(TGA)、震动样品磁强计(VSM)和zeta电位仪等对制得的MNP-CS进行表征.结果表明,壳聚糖成功地被包裹在磁性纳米颗粒表面.颗粒可完全分散于pH=1~14的水溶液中,形成均匀溶胶,Fe3O4磁核能抵抗0.1 mol/L的HCl溶液腐蚀.MNP-CS颗粒直径为10~20 nm,饱和磁化值为50.05 emu/g,剩磁和矫顽力近似为零,具有明显的超顺磁性.制得的磁性纳米粒子吸附BSA时呈明显的S型等温吸附特性,单层吸附量约为300 mg/g.
A chitosan-coated magnetic nanoparticle was prepared and its characterization and protein adsorption properties were studied.Firstly, surface carboxyl functionalized magnetic nanoparticles (MNP-COOH) were prepared by co-precipitation method, The interaction of chitosan self-assembled in the MNPCOOH particle surface, in the case of formaldehyde to protect the amino group, the use of epichlorohydrin cross-linked hydroxyl groups on the surface of chitosan, after the completion of cross-linked deprotected amino protective agent, the surface Amino-rich chitosan-coated magnetic nanoparticles (MNP-CS) were synthesized and characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) TGA), vibrating sample magnetometer (VSM) and zeta potentiometer were used to characterize the prepared MNP-CS.The results showed that the chitosan was successfully encapsulated on the surface of the magnetic nanoparticles.The particles could be completely dispersed at pH = 1 ~ 14 aqueous solution to form a uniform sol, Fe3O4 magnetic core can resist the corrosion of 0.1 mol / L HCl solution.MNP-CS particles with a diameter of 10 ~ 20 nm, saturation magnetization value of 50.05 emu / g, remanence and coercivity similar Is zero, with obvious superparamagnetic absorption of the prepared magnetic nanoparticles BSA showed a S-shaped isotherm characteristics monolayer adsorption of about 300 mg / g.