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建立了HPLC法测定人唾液中的富组蛋白5。采用Varitide~(TM)RPC柱,以0.05%磷酸-乙腈为流动相,梯度洗脱,检测波长215 nm。富组蛋白5在100～2 000μg/ml浓度范围内线性关系良好,平均回收率为97.4%,RSD为1.21%。
A HPLC method was developed for the determination of Histone 5 in human saliva. The column of Varitide ~ (TM) RPC was eluted with 0.05% phosphoric acid-acetonitrile as mobile phase with the detection wavelength of 215 nm. Histone 5 had good linearity in the concentration range of 100-2 000 μg / ml with an average recovery of 97.4% and a RSD of 1.21%.