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In this study,the electrochemiluminescent (ECL) behavior of Nickel(Ⅱ) tetrasulfophthalocyantine (NiTSPc)/H2O2 on a heated indium tin oxide (ITO) electrode was investigated.The effect of pH value,electrochemical scan mode,concentration of NiTSPc and electrode surface temperature on the ECL intensities had been studied in detail.Based on the fact that the ECL of NiTSPc can be greatly enhanced by hydrogen peroxide at the ITO electrode,a new ECL biosensor for hydrogen peroxide has been developed.The possible mechanism for the ECL of NiTSPc has also been proposed.