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目的 :探讨纯钛表面二氧化钛(TiO_2)纳米管改性后对人牙周膜干细胞(human periodontal ligament stem cells,hPDLSCs)增殖、成骨分化能力的影响。评价TiO_2纳米管改性作为种植体表面改性方法的作用。方法:将实验组钛片进行阳极氧化TiO_2纳米管处理,对照组仅进行抛光处理。将hPDLSCs与两组钛材分别复合培养,检测其增殖及成骨相关蛋白的表达水平。结果:成功在钛材表面制备了多孔、有序、管径约为100 nm的TiO_2纳米管;实验组hPDLSCs较对照组1 d的增殖活性及4 d的ALP活性没有明显差异;而4、7 d实验组细胞的增殖活性低于对照组;而7 d、14 d及21 d实验组细胞ALP活性高于对照组;21 d时,实验组Col-I、OCN、Runx-2的基因表达水平明显高于对照组。结论:通过阳极氧化可在钛材表面制备多孔有序的TiO_2纳米管层;且TiO_2纳米管能有效促进hPDLSCs在钛表面的骨向分化。
OBJECTIVE: To investigate the effects of modified titanium dioxide (TiO 2) nanotubes on the proliferation and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs). Evaluate the effect of TiO 2 nanotube modification as a method of surface modification of implants. Methods: The experimental group was treated with anodized TiO 2 nanotubes and the control group was polished only. The hPDLSCs were cultured with two groups of titanium respectively to detect the proliferation and osteogenesis-related protein expression. Results: The porous and ordered TiO 2 nanotubes with a diameter of about 100 nm were successfully prepared on the surface of titanium. The proliferative activity of hPDLSCs in the experimental group and the ALP activity at 4 d were not significantly different from those of the control group. However, The experimental group’s cell proliferation activity was lower than that of the control group. On the 7th, 14th and 21st day, the ALP activity of the cells in the experimental group was higher than that of the control group. On the 21st day, the gene expression of Col-I, OCN and Runx- Obviously higher than the control group. CONCLUSION: Porous and ordered TiO 2 nanotubes can be prepared on the surface of titanium by anodic oxidation. TiO 2 nanotubes can effectively promote the osteogenic differentiation of hPDLSCs on the surface of titanium.