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实验证实,兔胚胎从桑椹期扩张到胚泡的培养过程需要维生素。桑椹胚在含有11种水溶性维生素(生物素、泛酸、胆碱、肌醇、烟酰胺、维生素B_6、维生素B_2,维生素B_1、叶酸、维生素B_(12)和硫辛酸)的F_(10)培养液或在除去其中任一种维生素的培养液中培养5天后,测定胚泡直径。统计结果发现,除去肌醇,维生素B_6、维生素B_2和烟酰胺的胚泡扩张的直径显著减少。除去维生素B_(12)的胚泡扩张的直径明显增加,表明F_(10)培养液中维生素B_(12)的现有浓度对兔胚泡是有毒害的。
Experiments confirmed that rabbit embryos from mulberry stage expansion to blastocyst culture process requires vitamins. Mulberry embryos were cultured in F_ (10) medium containing 11 kinds of water-soluble vitamins (biotin, pantothenic acid, choline, inositol, nicotinamide, vitamin B_6, vitamin B_2, vitamin B_1, folic acid, vitamin B_ (12) and lipoic acid) After incubation in a liquid or in a culture solution in which any one of the vitamins was removed, the blastocyst diameter was measured. The statistical results showed that the diameter of blastocyst expansion with inositol, vitamin B_6, vitamin B_2 and niacinamide was significantly reduced. The diameter of blastocyst expansion excluding vitamin B_ (12) increased significantly, indicating that the existing concentration of vitamin B_ (12) in F_ (10) culture medium was toxic to rabbit blastocysts.