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目的:探讨火把化根片对实验性自身免疫性脑脊髓炎(EAE)大鼠的治疗作用及VCAM-1的影响。方法:模型组采用豚鼠髓鞘蛋白匀浆和福氏完全佐剂诱发大鼠急性EAE;治疗组在模型组基础上予以火把化根片(600mg.kg-1.d-1,日2次口服),观察临床表现并评分;HE染色和Loyez氏髓鞘染色观察病理和髓鞘改变;免疫组化技术测定脑和脊髓的VCAM-1水平。结果:治疗组未出现临床症状;VCAM-1为(2.13±0.99),与模型组比较P<0.05;脑和脊髓小血管周围炎症细胞浸润明显减少;髓鞘结构完整。结论:火把化根片对EAE有治疗作用,其作用机制与抑制VCAM-1表达有关。
Objective: To investigate the therapeutic effect of “Fuzhuguan” tablet on experimental autoimmune encephalomyelitis (EAE) rats and the effect of VCAM-1. Methods: The acute EAE was induced by the guinea pig myelin protein homogenate and Freund’s complete adjuvant in the model group. The treatment group was given the bee venom tablet (600mg.kg-1.d-1, 2 times daily on the basis of the model group) ). The clinical manifestations were observed and scored. The pathological and myelin changes were observed by HE staining and Loyez’s myelin staining. The levels of VCAM-1 in brain and spinal cord were determined by immunohistochemistry. Results: There was no clinical symptom in the treatment group; VCAM-1 was (2.13 ± 0.99), compared with the model group (P <0.05); infiltration of inflammatory cells around the small vessels in the brain and spinal cord was significantly reduced; myelin sheath structure was intact. CONCLUSION: PTZ has a therapeutic effect on EAE and its mechanism is related to the inhibition of VCAM-1 expression.