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目的:阐述LHA和PVN间nesfatin-1神经通路的构成,探讨PVN nesfatin-1对胃收缩幅度及频率的影响及潜在机制。方法:采用荧光金逆行追踪结合荧光免疫组化技术,观察LHA-PVN间nesfatin-1神经通路构成;采用细胞外放电记录,观察nesfatin-1对GD放点活动的影响;通过在体胃运动技术,观察nesfatin-1对清醒自由活动大鼠胃收缩幅度和频率的影响。结果:Nesfatin-1能够抑制GD-E神经元放电(1.97±0.12 Hz vs.1.15±0.07 Hz)促进GD-I神经元放电(1.74±0.10 Hz vs.3.04±0.18 Hz),H4928能够部分阻断nesfatin-1对GD神经元(GD-E:1.38±0.08 Hz,P<0.05 vs.nesfatin-1;GD-I:2.49±0.15 Hz,P<0.05 vs.nesfatin-1)的影响;PVN内微量注射nesfatin-1能够抑制大鼠胃运动,呈量效依赖关系;LHA和PVN间有nesfatin-1神经纤维联系;电刺激LHA后,GD神经元放电频率增加(GD-E:2.06±0.12 Hz vs.4.23±0.21 Hz,GD-I:1.61±0.09 Hz vs.4.83±0.25 Hz),预先向PVN注射抗NUCB2/nesfatin-1抗体后,GD-E神经元放电频率减弱(4.91±0.25 Hz vs.4.23±0.21 Hz),而GD-I神经元放电频率增强(4.15±0.18 Hz vs.4.83±0.25)。结论:PVN内nesfatin-1可调控大鼠GD神经元放电活动及胃运动,该效应受LHA调控。
OBJECTIVE: To elucidate the structure of nesfatin-1 neural pathway between LHA and PVN, and to investigate the effect of nepsfatin-1 on the amplitude and frequency of gastric contraction and its potential mechanism. Methods: Fluorescent gold retrograde tracing combined with fluorescence immunohistochemistry was used to observe the nesfatin-1 neural pathway in LHA-PVN. The extracellular discharge recording was used to observe the effect of nesfatin-1 on GD locomotor activity. To observe the effect of nesfatin-1 on gastric contraction amplitude and frequency in awake free-moving rats. Results: Nesfatin-1 could inhibit GD-I neurons from firing (1.97 ± 0.12 Hz vs.1.15 ± 0.07 Hz), which could cause GD-I neurons to discharge (1.74 ± 0.10 Hz vs.3.04 ± 0.18 Hz) The effect of nesfatin-1 on GD neurons (GD-E: 1.38 ± 0.08 Hz, P <0.05 vs. nesfatin-1; GD-I: 2.49 ± 0.15 Hz, P <0.05 vs. nesfatin-1) Nesfatin-1 could inhibit the gastric motility in rats in a dose-dependent manner. There was nesfatin-1 nerve fiber connection between LHA and PVN. The discharge frequency of GD neurons was increased after electrical stimulation of LHA (GD-E: 2.06 ± 0.12 Hz vs .4.23 ± 0.21 Hz, GD-I: 1.61 ± 0.09 Hz vs.4.83 ± 0.25 Hz). After pre-injection of PVN with anti-NUCB2 / nesfatin-1 antibody, GD-E neurons had a weaker firing frequency (4.91 ± 0.25 Hz vs. 4.23 ± 0.21 Hz), while the discharge frequency of GD-I neurons increased (4.15 ± 0.18 Hz vs 4.83 ± 0.25). Conclusion: nesfatin-1 in PVN can regulate the discharge activity and gastric motility of GD neurons in rats, which is regulated by LHA.