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【目的】研究不同浓度CaCl2处理与提高不同树龄库尔勒香梨树抗寒性的关系。【方法】以5年生、15年生、25年生库尔勒香梨树为试验材料,在展叶期(2012年5月5日)第一次喷施10、15、20、25 mmol/L浓度的CaCl2,每隔20 d喷施一次,共喷施三次,在休眠期枝条取样,在人工低温胁迫下测定膜透性和脯氨酸含量。【结果】随着低温胁迫温度的降低,不同浓度CaCl2处理后,香梨树1年生枝条的膜透性、脯氨酸的含量有不同程度的增加;5年、25年生库尔勒香梨树20 mmol/L CaCl2处理时,15年生库尔勒香梨树10 mmol/L CaCl2处理时可有效的提高膜保护能力,提高脯氨酸的含量。5年生库尔勒香梨树脯氨酸含量累积量最多,高出15年生的香梨树脯氨酸累积量的78.78%,增加幅度为5.86倍,膜透性增加幅度为3.85倍。15年生香梨树膜透性增加幅度为3.11倍,脯氨酸含量增加幅度为79.05%。25年生香梨树膜透性增加幅度为2.58倍,脯氨酸含量增加幅度为88.64%。【结论】喷施CaCl2可以提高库尔勒香梨树的抗寒性。研究是以不同生长期叶面喷施进行的,而且人工低温胁迫下测定枝条的抗寒性时离母体进行,今后不同试验方法对提高抗寒性需要进一步深入研究。
【Objective】 The aim of this study was to investigate the effects of different concentrations of CaCl2 treatment on the cold resistance of Korla fragrant pear trees of different ages. 【Method】 Five-year-old, 15-year-old and 25-year-old Korla fragrant pear were used as test materials and the first spraying of CaCl2 (10,15,20,25 mmol / L) , Sprayed once every 20 days and sprayed three times in total. During the dormant period, the branches were sampled and the membrane permeability and proline content were determined under artificial low temperature stress. 【Result】 With the decreasing of temperature, the membrane permeability and proline content of 1-year-old branches of pear increased with the different concentrations of CaCl2 treatment. In 5 years, the 25-year-old Korla pear 20 mmol / L CaCl2 treatment, 15-year-old Korla fragrant pear 10 mmol / L CaCl2 treatment can effectively improve the membrane protection, increase proline content. The 5-year-old Korla fragrant pear had the largest accumulation of proline content, which was 78.78% higher than that of the 15-year-old pear, which increased by 5.86 times and the membrane permeability increased by 3.85 times. 15-year-old rose pear membrane permeability increased by 3.11 times, proline content increased by 79.05%. Permeability of 25-year-old pear rose by 2.58-fold and proline content increased by 88.64%. 【Conclusion】 The spraying of CaCl2 can improve the cold resistance of Korla fragrant pear. The study was conducted at different growth stages of foliar spraying, and cold resistance of artificial branches under cold stress was measured from the parent body. Different test methods should be further studied to improve the cold resistance in the future.