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目的:探讨CpG-ODN体外对HBV复制和HBV抗原表达的抑制作用。方法:CpG-ODN体外刺激人外周血单个核细胞(PBMC),用ELISA检测培养液中IFN-α及IFN-γ的水平。将不同比例的PBMC培养上清与HepG2. 2. 15细胞共孵育 2、4、6、8d后,用ELISA和荧光定量PCR法,分别检测培养上清液中HB sAg、HBeAg和HBVDNA的水平。结果:CpG-ODN可诱导PB MC分泌IFN-α和IFN-γ。CpG-ODN本身虽不能直接抑制HBV的复制,但经刺激活的化的PBMC的培养上清,却能显著抑制 2. 2. 15细胞产生HBsAg、HBeAg和HBVDNA,在培养的第 8天,抑制率最高分别达 90. 8%、31. 3%和 32. 2%。结论:CpG-ODN作为一种新型的免疫调节剂,可诱发机体的免疫效应,抑制HBV复制和HBV抗原的表达。
Objective: To investigate the inhibitory effect of CpG-ODN on HBV replication and HBV antigen expression in vitro. Methods: Human peripheral blood mononuclear cells (PBMCs) were stimulated with CpG-ODN in vitro. The levels of IFN-α and IFN-γ in the culture fluid were detected by ELISA. Different concentrations of PBMC culture supernatants were incubated with HepG2.2.15 cells for 2, 4, 6 and 8 days. The levels of HBsAg, HBeAg and HBVDNA in culture supernatants were detected by ELISA and fluorescence quantitative PCR respectively. Results: CpG-ODN induced PBMC to secrete IFN-α and IFN-γ. Although CpG-ODN itself can not directly inhibit the replication of HBV, the culture supernatant of stimulated PBMC can significantly inhibit 2. 2. 15 cells to produce HBsAg, HBeAg and HBVDNA, on the 8th day of culture, inhibition The highest rates were 90.8%, 31.3% and 32.2% respectively. Conclusion: As a novel immunomodulatory agent, CpG-ODN can induce the immune response and inhibit the HBV replication and HBV antigen expression.