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为测定在胆酸盐和脱氧胆酸盐存在下氨提配位剂的加质子常数和钙配合物稳定常数,对常规电住法做了一些修正。用所建立的方法测定了EDTA、DTPA、EGTA及NTA的有关常数及其随胆汁酸浓度的变化。
In order to determine the proton-increasing proton and calcium complex stability constants in the presence of cholate and deoxycholate, some modifications were made to the conventional electroblotting method. The established methods were used to determine the relative constants of EDTA, DTPA, EGTA and NTA and their changes with bile acid concentration.