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AIM:To study the therapeutic effects of anti-fibrosis herbsand selenium on hepatic fibrosis induced by carbontetrachloride (CCl_4) in rats and the underlining molecularmechanisms.METHODS:Fifty-three Wistar rats were randomly dividedinto:normal control group,model control group,colchicinegroup,anti-fibrosis herbs group (AF group) and anti-fibrosisherbs plus selenium group (AS group).The last four groupswere administered with CCl_4 at the beginning of experimentto induce hepatic fibrosis.Then colchicine,anti-fibrosis herbsand selenium were used to treat them.The normal controlgroup and the model control group were given normal salineat the same time.At the end of the 6~(th) week,rats in eachgroup were sacrificed.Blood and tissue specimens weretaken.Serum indicators (ALT,AST,HA,LN) were determinedand histopathological changes were graded.Lymphocyte CD_4and CD_8 were examined by flow cytometry.Expression ofTGF-β_1 and NF-κB was detected by immunohistochemistryand expression of TGF-β_1 mRNA was detected by semi-quantified RT-PCR.RESULTS:Histological grading showed much a smallerdegree of hepatic fibrogenesis in AS group and AF groupthan that in colchicine group and model control group.Theserum content of ALT,AST,HA and LN in AF group and ASgroup were significantly lower than that in colchicine group(ALT:65.8±26.5,67.3±18.4 and 96.2±20.9 in AF,AS andcolchicine groups respectively;AST:150.8±34.0,154.6±27.3and 215.8±24.6 respectively;HA:228±83,216±58 and416±135 respectively;LN:85.9±15.0,80.6±18.6 and106.3±14.2 respectively) (P<0.05).The level of CD_4 andCD_4/CD_8 ratio in AF group and AS group was significantly higherthat those in cochicine group (CD_4:50.8±3.8,52.6±3.4 and40.2±2.1 in AF,AS and colchicine groups respectively;CD_4/CD_8 ratio:1.45,1.46 and 1.26,respectively (P<0.05).The expression level of NF-κB and TGF-β_1 in the liver tissuesof AF and AS treatment groups was markedly decreasedcompared with that in cochicine group,and TGF-β_1 mRNAwas also markedly decreased (1.07±0.31 and 0.98±0.14 vs2.34±0.43,P<0.05).CONCLUSION:Anti-fibrosis herbs and selenium havebeneficial effects on hepatic fibrosis in rats by enhancingimmunity and inhibiting NF-κB and TGF-β_1 expressions.
AIM: To study the therapeutic effects of anti-fibrosis herbs and selenium on hepatic fibrosis induced by carbontetrachloride (CCl 4) in rats and the underlining molecular mechanisms. METHODS: Fifty-three Wistar rats were randomly dividedinto: normal control group, model control group, colchicinegroup, anti-fibrosis herbs group (AF group) and anti-fibrosisherbs plus selenium group (AS group) .The last four groupswere administered with CCl_4 at the beginning of experiment to induce hepatic fibrosis.Then colchicine, anti-fibrosis herbs and selenium were used to treat them The normal controlgroup and the model control group were given normal salineat the same time. At the end of the 6th (th) week, rats in eachgroup were sacrificed.Blood and tissue specimens weretaken. Serum indicators (ALT, AST, HA, LN) were determined and histopathological changes were graded. Lymphocyte CD_4 and CD_8 were examined by flow cytometry. Expression of TGF-β_1 and NF-κB was detected by immunohistochemistry and expression of TGF-β_1 m RNA was detected by semi-quantified RT-PCR .RESULTS: Histological grading showed much smaller lesion of hepatic fibrogenesis in AS group and AF groupthan that in colchicine group and model control group. The content of ALT, AST, HA and LN in AF group and AS groups were significantly lower than those in colchicine groups (ALT: 65.8 ± 26.5, 67.3 ± 18.4 and 96.2 ± 20.9 in AF, AS andcolicine groups respectively; AST: 150.8 ± 34.0, 154.6 ± 27.3 and 215.8 ± 24.6 respectively; HA: 228 ± 83,216 ± 58 and 416 ± 135 respectively; LN: 85.9 ± 15.0, 80.6 ± 18.6 and 106.3 ± 14.2 respectively) (P <0.05). The level of CD_4 and CD_4 / CD_8 ratio in AF group and AS group was significantly higherthat those in The cochicine group (CD_4: 50.8 ± 3.8, 52.6 ± 3.4 and 40.2 ± 2.1 in AF, AS and colchicine groups respectively; CD_4 / CD_8 ratio: 1.45, 1.46 and 1.26, κB and TGF-β_1 in the liver tissues of AF and AS treatment groups were markedly decreased with that in cochicine group, and TGF-β_1 mRNA was als o markedly decreased(1.07 ± 0.31 and 0.98 ± 0.14 vs 2.34 ± 0.43, P <0.05) .CONCLUSION: Anti-fibrosis herbs and selenium have beneficial effects on hepatic fibrosis in rats by enhancing immunity and inhibiting NF-κB and TGF-β_1 expressions.