目的　建立MBP特异性自身反应性T细胞克隆 ,为进一步探讨EAE的发病机制和治疗奠定基础。方法　以MBP肽免疫C5 7BL 6小鼠 ,取其淋巴结细胞 ,加同系鼠脾细胞体外共同培养于含IL 2的培养液中 ,并以MBP肽反复刺激。收集培养细胞 ,以流式细胞仪进行鉴定。结果　流式细胞仪分析显示 ,90 %以上的细胞为CD4+ T细胞。结论　成功地建立了C5 7BL 6小鼠的MBP反应性T细胞克隆 ,该细胞克隆为MBP特异性自身反应性CD4+ T细胞。 OBJECTIVE: To establish MBP-specific autoreactive T cell clones and lay a foundation for further exploring the pathogenesis and treatment of EAE. Methods C5 7BL 6 mice were immunized with MBP peptide and their lymph node cells were harvested. Co-cultures of spleen cells from co-cultures were cultured in culture medium containing IL 2 and stimulated repeatedly with MBP peptide. The cultured cells were collected and identified by flow cytometry. Results Flow cytometry analysis showed that more than 90% of the cells were CD4 + T cells. Conclusion MBP-reactive T cell clones of C5 7BL 6 mice were successfully established and cloned as MBP-specific autoreactive CD4 + T cells.