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目的:以人正常绒毛组织和人绒毛癌细胞JAR为研究对象,探求ERCC2表达与人绒毛癌细胞JAR发生的关系。方法:分别取人正常绒毛组织100mg,人绒毛癌细胞JAR108,提取组织中总RNA,采用RT-PCR的方法分析细胞中ERCC2表达水平。结果:ERCC2在人绒毛癌细胞JAR中表达显著低于人正常绒毛组织。结论:ERCC2在人绒毛癌细胞JAR中低表达可能是人绒毛癌细胞JAR恶变形成的基础。
OBJECTIVE: To investigate the relationship between the expression of ERCC2 and the occurrence of JAR in human choriocarcinoma using the human normal villus tissue and human choriocarcinoma JAR as research object. Methods: Human normal human villi carcinoma cell line JAR108 was harvested from 100 mg of normal human villi and total RNA was extracted. The expression of ERCC2 was analyzed by RT-PCR. Results: The expression of ERCC2 in human Vulvar carcinoma cells JAR was significantly lower than that in normal human villi. Conclusion: The low expression of ERCC2 in human choriocarcinoma JAR may be the basis of JAR malignant transformation in human choriocarcinoma cells.