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目的探讨慢性应激对小鼠情绪行为以及血清IFN-γ、IL-4水平的影响。方法选取BALB/c小鼠50只,随机分为正常对照组和应激组,每组25只。采用自制的束缚制动方法建立慢性心理应激模型,应激后进行旷场实验和悬尾实验,摘眼球取血采用ELISA方法检测血清IFN-γ和IL-4的水平。结果应激组小鼠在旷场实验中的中央格停留时间显著延长(P<0.01),探究活动显著减少(P<0.01),粪便次数显著增多(P<0.01);悬尾实验中,应激组小鼠不动时间显著延长(P<0.01);应激组小鼠血清IFN-γ水平明显低于对照组(P<0.05),IL-4水平与对照组相比差异无统计学意义(P>0.05)。结论慢性心理应激可以导致机体行为发生改变,出现焦虑、抑郁情绪,同时降低机体的免疫功能。
Objective To investigate the effects of chronic stress on emotional behavior, serum levels of IFN-γ and IL-4 in mice. Methods Fifty BALB / c mice were randomly divided into normal control group and stress group, with 25 rats in each group. Chronic psychological stress model was established by self-made restraint braking method. After open stress test, open-field test and tail-suspension experiment were carried out. Erythrocytes were collected and the levels of IFN-γ and IL-4 were measured by ELISA. Results In the open-field test, the median residence time of rats in stress group was significantly longer (P <0.01), significantly lower in exploration activity (P <0.01) and significantly increased number of stools (P <0.01) (P <0.01). The level of IFN-γ in the stress group was significantly lower than that in the control group (P <0.05). There was no significant difference in IL-4 level between the challenge group and the control group (P> 0.05). Conclusion Chronic psychological stress can lead to changes in the behavior of the body, anxiety and depression, and reduce the body’s immune function.