Meisoindigotin has been demonstrated as a new type of cancer chemotherapeutic agent.N-11C-meisoindigotin was synthesized by N-11C-methylation of the isoindigotin precursor with 11C-labelled methyl triflate.The decay corrected radiochemical yields were 15-25％,and the specific radioactivity was 1.0-1.2 Ci/μmol at the end of synthesis.The cellular uptake of [N-11C]-meisoindigotin was evaluated in four different lung cancer cell lines.Our results showed that the A549,GLC-82,95D cell lines exhibited higher uptake than 95C cell line after incubation for 60 min.N-11C-meisoindigotin was a promising candidate for further development as a novel PET radiotracer for imaging of cyclin-dependent kinases (CDKs) and GSK-3β.