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目的观察急性大剂量华支睾吸虫感染大鼠模型中的肝脏病理形态学和血清相关指标的改变。方法 12只5~6周龄雌性Sprague-Dawley(SD)大鼠分为实验组和不感染对照组。实验组灌胃200个华支睾吸虫囊蚴/大鼠,采用改良加藤法检查粪便中虫卵。灌胃5周后剖杀,取肝脏,切片,HE染色后观察病理改变。剖杀前采血,分离血清,检测总胆红素(TBIL)、间接胆红素(DBIL)、碱性磷酸酶(ALP)、谷草转氨酶(AST)、谷丙转氨酶(ALT)、白细胞介素-6(IL-6)、肿瘤坏死因子(TNF-α)含量,用华支睾吸虫IgG抗体试剂盒检测感染特异性。结果实验组大鼠感染囊蚴30d左右粪便中均查到华支睾吸虫虫卵;肉眼见肝脏肿大、淤血,胆管堵塞明显,HE显示胆管扩张、管壁增厚明显,肝细胞肿胀,部分肝小叶结构破坏,胆管上皮细胞排列不规则水肿、坏死;血清TBIL、DBIL、ALP、AST、ALT、IL-6、TNF-α水平较对照组均显著增高(P<0.05),并产生高水平的华支睾吸虫特异性IgG抗体。结论急性大剂量华支睾吸虫感染时肝脏病理形态学和血清指标显著性改变,表明存在严重的肝脏损伤,应予以重视。本研究对探讨人体急性感染华支睾吸虫的致病性具有一定借鉴。
Objective To observe the pathological changes of liver pathology and serum related indexes in the rat models of acute and high doses of Clonorchis sinensis infection. Methods Twelve female Sprague-Dawley (SD) rats aged 5-6 weeks were divided into experimental group and non-infected control group. The experimental group was orally administered with 200 Clonorchis sinensis metacercariae / rats, and the improved Kato method was used to examine the eggs in the feces. After 5 weeks of gavage, they were sacrificed and the liver and slices were taken. The pathological changes were observed after HE staining. Serum samples were taken before severing and the serum was separated for determination of total bilirubin (TBIL), indirect bilirubin (DBIL), alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), interleukin- 6 (IL-6) and tumor necrosis factor-α (TNF-α) were detected. The infection specificity was detected by using Clonorchis sinensis IgG antibody kit. Results In the experimental group, C. sinensis eggs were found in the feces of metacercariae infected for about 30 days. The liver of the experimental group was swollen, the congestion was obvious, the obstruction of the bile duct was obvious. HE showed the dilatation of the bile ducts, obvious wall thickening and swelling of hepatocytes. The structure of hepatic lobules was destroyed and the bile duct epithelial cells were irregularly edematous and necrotic. The levels of serum TBIL, DBIL, ALP, AST, ALT, IL-6 and TNF-αwere significantly higher than those in control group (P <0.05) Clonorchis sinensis specific IgG antibody. Conclusions Acute and high doses of Clonorchis sinensis infection of liver pathological morphology and serum indicators significantly changed, indicating that there is a serious liver injury, should be taken seriously. This study has some reference to explore the pathogenicity of human acute infection Clonorchis sinensis.