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目的探讨GNB3基因C825T多态性与新疆和田地区维吾尔族老人长寿的关系。方法采用聚合酶链反应-限制性片段长度多态法(PCR-RFLP)和直接测序法检测百岁组(年龄不小于100岁)65例和长寿组(90～99岁)100例老人GNB3基因825位点的基因型,对照组112例(年龄不超过70岁自然死亡人群)作为对照组。结果百岁组和对照组GNB3825C/T的基因型和等位基因频率分布差异均具有统计学意义(P=0.0021,P<0.0001)。其中百岁组CC基因型的频率为60.0%,对照组为36.6%(OR2.60;95%CI1.39～4.89)。结论本研究初步表明,新疆和田地区维吾尔族人GNB3基因C825T多态性与个体寿命密切相关,但同时也应考虑长寿是年龄依赖的多种因素影响的结果。
Objective To investigate the relationship between GNB3 gene C825T polymorphism and Uigur elderly in longevity in Hetian area of Xinjiang. Methods 65 cases of centenarians (age not less than 100 years old) and 100 elderly people of longevity group (90-99 years old) were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct sequencing The genotype of 825 locus and the control group of 112 cases (no more than 70-year-old natural deaths) served as the control group. Results The genotype and allele frequencies of GNB3825C / T in centenarian group and control group were all significantly different (P = 0.0021, P <0.0001). Among them, the frequency of CC genotype in centenarian group was 60.0% and that in control group was 36.6% (OR2.60; 95% CI 1.39 ~ 4.89). Conclusions Our study shows that the polymorphism of GNB3 gene C825T in Uyghur people in Hetian region is closely related to the longevity of individuals. However, longevity should also be considered as a result of various age-dependent factors.