论文部分内容阅读
目的 检测大鼠脑缺血再灌注(I/R)后海马神经细胞损伤及炎症因子的变化,同时比较海马DG、CA1和CA3三亚区炎症因子白细胞介素1β(IL-1β)、IL-6和肿瘤坏死因子α(TNFα)的表达变化.方法 通过线栓法制备SD大鼠大脑中动脉栓塞(MCAO)的I/R模型,实验动物随机分为假手术组(SHAM组)和MCAO再灌组(MCAO组).苏木精-伊红染色法检测大鼠大脑海马上述3亚区的细胞形态改变.免疫荧光法检测炎症因子IL-1β、IL-6和TNFα的表达水平.结果 与SHAM组比较,MCAO组大鼠海马的上述3亚区神经细胞损伤严重,炎性细胞浸润,神经细胞凋亡,且各亚区的IL-1β、IL-6和TNFα表达显著增加,同时MCAO组CA1亚区各炎症因子的表达均高于DG和CA3亚区表达,差异具有统计学意义(P<0.05).结论 I/R可导致大鼠海马上述3亚区的神经细胞损伤,炎性细胞浸润,神经细胞凋亡,且导致炎症因子释放增加,同时MCAO组动物海马CA1亚区炎症因子表达显著高于DG和CA3亚区,提示CA1亚区对I/R损伤可能更加敏感.“,”Objective To detect the damage of hippocampal neurons and the changes in inflammatory cytokines in rats after cerebral ischemia-reperfusion(I/R)and compare the expressions of IL-1β,IL-6 and TNFαin hippocampal DG,CA1 and CA3 subregions.Methods The focal cerebral I/R model was induced by an intraluminal filament embolism.The SD rats were randomly divided into the sham-operated group(SHAM group)and the middle cerebral artery occlusion-reperfusion group(MCAO group).HE staining was employed to detect the damage to hippocampal DG, CA1 and CA3 subregions.The expression levels of IL-1β, IL-6 and TNFα were detected by immunofluorescence assay.Results Compared with SHAM group,hippocampal DG,CA1 and CA3 subregion neurons in MCAO group were severely damaged, with occurred inflammatory cell infiltration,and a large amount of neurons apoptosis, and the expressions of IL-1β, IL-6 and TNFαin each subregion increased significantly.At the same time, in MCAO group, the expression of inflammatory cytokines in CA1 subregion was more significant than that in DG and CA 3 subregions(P<0.05).Conclusion Cerebral I/R could cause neuronal damage, inflammatory cell infiltration, and neuronal apoptosis in the DG, CA1 and CA3 subregions of the hippocampus and increase the release of inflammatory cytokines.In MCAO group, the expression of inflammatory cytokines in CA1 subregion of hippocampus is significantly higher than that in DG and CA 3 subregions, suggesting that CA1 region is more sensitive to I/R injury.