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目的：研究抑癌基因Ｐ２１ 、Ｐ５３ 在急性淋巴细胞性白血病中的蛋白表达水平。方法：应用Ｗｅｓｔｅｒｎ Ｂｌｏｔ 及Ｓ－ Ｐ 免疫组织化学染色技术检测抑癌基因Ｐ２１ 、Ｐ５３ 在ＡＬＬ 中的蛋白表达水平。结果：１０ ．７１ ％ ＡＬＬ 患者Ｐ５３ 蛋白表达异常且表达水平有所增高，但与对照组比较无显著性差异（ Ｐ＞ ０ ．０５） 。在２８ 例ＡＬＬ 患者中未检测到Ｐ２１ 蛋白表达缺失及表达水平异常，与对照组比较无显著性差异（ Ｐ＞ ０ ．０５） 。结论：至少在蛋白表达水平，Ｐ２１ 与ＡＬＬ 的发生及演变过程无明显相关性；Ｐ５３ 蛋白表达在ＡＬＬ 中有异常改变，但无统计学意义，尚需进一步研究证实。
Objective: To study the protein expression levels of tumor suppressor genes P21 and P53 in acute lymphoblastic leukemia. METHODS: Western blot and S-P immunohistochemical staining were used to detect the protein expression levels of tumor suppressor genes P21 and P53 in ALL. Result: 10. P53 protein expression was abnormal in 71% ALL patients and the expression level was increased, but there was no significant difference compared with the control group (P> 0.05). No loss of P21 protein expression and abnormal expression levels were detected in 28 ALL patients. There was no significant difference from the control group (P > 0.05). Conclusion: At least at the level of protein expression, there is no significant correlation between the occurrence and evolution of P21 and ALL; P53 protein expression has abnormal changes in ALL, but there is no statistical significance, and further studies are needed to confirm.