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利用离子色谱-脉冲安培检测器对当归补血汤中单糖(阿拉伯糖、葡萄糖、果糖、核糖)和蔗糖的测定方法进行了研究。采用Carbopac PA10分离柱和脉冲安培检测器,选择0.055 mol/L Na OH作淋洗液,可使当归补血汤中单糖和蔗糖很好地分离。方法检出限在3.8~200.5μg/L范围,线性范围在:0.01~20 mg/L,加标回收率为90.03%~98.93%,RSD为0.19%~5.52%(n=6)。该方法简便快速、分离效果、灵敏度高、测定准确率高,适用于归补血汤中单糖和蔗糖的测定。
The method of determination of monosaccharide (arabinose, glucose, fructose, ribose) and sucrose in Dangguibuxue Decoction was studied by ion chromatography - pulse amperometric detector. Using Carbopac PA10 column and pulsed amperometric detector, 0.055 mol / L NaOH was selected as the eluent to separate well the monosaccharide and sucrose from Dangguibuxuetang. The detection limit was 3.8 ~ 200.5μg / L. The linear range was 0.01 ~ 20 mg / L. The recoveries were 90.03% ~ 98.93% with RSDs of 0.19% ~ 5.52% (n = 6). The method is simple and rapid, the separation effect, high sensitivity and high determination accuracy are suitable for the determination of monosaccharide and sucrose in Guixue Xue Tang.