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通过基于16S rRNA基因克隆文库的方法研究了天然气库上方土壤的微生物群落结构。针对大港油田板876气库上方同一取样点1.0 m和2.0 m的土壤样品构建了16S rDNA克隆文库DGS1和DGS2,并对其150个阳性克隆进行限制性酶切片段长度多样性分析(ARDRA)。结果表明,克隆文库DGS1有40个操作分类单元(OTU),克隆文库DGS2有39个OTU,该天然气库上方土壤中微生物较丰富;但由于土壤深度的不同两个土壤样品微生物群落结构存在着差异。每个OTU的代表克隆序列分析结果表明,克隆文库DGS1中优势菌群为芽单胞菌(Gemmatimonadetes)28%、绿弯菌(Chloroflexi)23%和放线菌(Actinobacterium)21%;克隆文库DGS2菌群分布较平均,其中Chloroflexi19%、硫氧化菌(Sulfur-oxidizing)12%、酸酐菌(Acidobacteria)10%、Gemmatimonadetes10%。天然气库上方土壤微生物多样性的分子分析可为开展微生物油气勘探(MPOG)技术奠定基础。
The microbial community structure of soils above natural gas pool was studied by a 16S rRNA gene cloning library. 16S rDNA clone libraries DGS1 and DGS2 were constructed for soil samples of 1.0 m and 2.0 m above the same sampling point above Pan 876 gas tank in Dagang Oilfield and restriction fragment length polymorphism analysis (ARDRA) was performed on 150 positive clones. The results showed that there were 40 operating taxonomic units (OTU) in the cloning library DGS1 and 39 OTU in the cloning library DGS2, which was rich in soil above the natural gas pool. However, there were differences in the microbial community structure between the two soil samples . The representative clone sequence analysis results of each OTU showed that the predominant colonies of DGS1 in the clone library were 28% of Gemmatimonadetes, 23% of Chloroflexi and 21% of Actinobacterium. The clone library DGS2 Microflora is distributed evenly, with Chloroflexi 19%, Sulfur-oxidizing 12%, Acidobacteria 10% and Gemmatimonadetes 10%. Molecular analysis of soil microbial diversity above natural gas reservoirs may provide the basis for the development of microbial oil and gas exploration (MPOG) technology.