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用从条斑紫菜EST数据库中筛选合成的微卫星引物对8个坛紫菜丝状体品系进行微卫星DNA指纹扩增。5个微卫星引物共扩增出32条带,其中3对引物所扩增出的5个条带(AU192094-127、AU187410-335、AU187410-190、AU194267-203和AU194267-328)被用来构建8个坛紫菜丝状体品系的DNA指纹。在这个图谱中,每个丝状体品系都有独一的指纹模式,彼此很容易被区分开。所获得的DNA指纹图谱,可用来进行孟德尔分离研究,及为坛紫菜纯系鉴定提供分子基础。
Microsatellite DNA fingerprinting of eight algae filamentous laver filamentous lines was amplified by screening the microsatellite DNA primers synthesized from the Porphyra yezoensis EST database. A total of 32 bands were amplified by 5 microsatellite primers, of which 5 bands (AU192094-127, AU187410-335, AU187410-190, AU194267-203 and AU194267-328) amplified by 3 pairs of primers were used Construction of DNA fingerprinting of eight algae filamentous filamentous strains. In this map, each filament has a unique fingerprint pattern that is easily distinguishable from each other. The DNA fingerprinting obtained can be used for Mendelian separation studies and provide the molecular basis for the identification of pure seaweed laver.