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吡咯-咪唑聚酰胺是一类人工合成的能够在B-DNA小沟特异性识别碱基序列的有机小分子,并且能通过细胞膜进入细胞,调控基因的表达。它主要由五元杂环化合物N-甲基吡咯(Py)、N-甲基咪唑(Im)、N-甲基-3-羟基吡咯(Hp)芳香氨基酸及脂肪链氨基酸组成。在这种小分子对生物大分子识别事件中,脂肪链作为构建聚酰胺及其结合物的一部分,在聚酰胺特异性识别DNA、延长DNA识别序列、连接分子荧光标记、对DNA指定位点的烷基化及基因调控等方面都起着非常重要的作用。本文阐述了脂肪链在上述诸方面应用的研究进展,并简要地分析了存在的问题和应用前景。
Pyrrole-imidazole polyamides are a class of small, synthetic, synthetic, small organic molecules that specifically recognize the base sequence in the minor groove of B-DNA and can enter the cell through the cell membrane to regulate gene expression. It is mainly composed of 5-membered heterocyclic compounds such as N-methylimidazole (Py), N-methyl-3-hydroxypyrrole (Hp) aromatic amino acid and fatty chain amino acid. In this small molecule recognition of biological macromolecules, fatty chain as part of the construction of polyamides and their conjugates, the specific recognition of DNA in the polyamide, extending the DNA recognition sequence, molecular fluorescent markers connected to the DNA designated sites Alkylation and gene regulation and so plays a very important role. This article expounds the research progress of the application of fatty chains in the above aspects and briefly analyzes the existing problems and application prospects.