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目的:探讨肝硬化患者外周血IP-10、IP-10mRNA的表达及其与转氨酶水平的关系。方法:筛选典型HBV感染后肝硬化患者,以ELISA法检测患者血清IP-10水平;Real-time PCR检测IP-10mRNA含量,以lg cDNA/lg GAPDH代表其mRNA水平。结果:肝硬化患者血清IP-10及PBMC中IP-10mRNA水平分别为(299.3±77.2)ng/L、0.7479±0.1495,与对照组相比差异有统计学意义(P<0.05,P<0.01);ALT、AST升高组外周血IP-10及其mRNA升高,与转氨酶正常组相比差异有统计学意义(P<0.05,P<0.01)。升高组中IP-10与ALT、AST及ALT/AST比值存在相关(r=0.6284,0.7162,0.6970,P<0.01)。结论:肝硬化患者外周血IP-10及其mRNA表达增高,与肝细胞损伤程度密切相关,在介导肝炎后肝硬化进展中起重要作用。
Objective: To investigate the expression of IP-10 and IP-10mRNA in peripheral blood of cirrhotic patients and its relationship with aminotransferase levels. Methods: Serum IP-10 levels were detected by ELISA in patients with cirrhosis after typical HBV infection. The expression of IP-10 mRNA was detected by Real-time PCR and the mRNA level was expressed by lg cDNA / lg GAPDH. Results: The levels of IP-10 mRNA in IP-10 and PBMC of patients with cirrhosis were (299.3 ± 77.2) ng / L and 0.7479 ± 0.1495, respectively, which were significantly different from those in control group (P <0.05, P <0.01) The levels of IP-10 and its mRNA in peripheral blood increased in ALT, AST group, and there was significant difference compared with normal group (P <0.05, P <0.01). Elevated IP-10 and ALT, AST and ALT / AST ratio (r = 0.6284,0.7162,0.6970, P <0.01). Conclusion: The expression of IP-10 and its mRNA in peripheral blood of patients with cirrhosis is increased, which is closely related to the degree of hepatocyte injury and plays an important role in the progression of cirrhosis after hepatitis.