论文部分内容阅读
表面增强拉曼光谱技术是近年来快速发展的一种痕量特征标记性物质检测技术,达到了分子识别水平,具有很高的灵敏度,能够精确地分析和了解物质结构和成分。人体唾液中含有大量体征相关的特异性蛋白质和分泌物质。唾液检测具有无创、获取方便、适合开展大规模普查等优点。表面增强拉曼光谱检测技术可以快速地对唾液进行检测,二者的结合,有可能成为一种癌症诊断的新方法。本文利用表面增强光谱技术对肝癌患者进行实验研究,通过对肝癌患者唾液的采集、样品的处理、光谱的采集及光谱分析,肝癌特异性标记物AFP(alpha fetoprotein)的检测及分析等过程得到肝癌相关特征信息,表明肝癌患者与正常人的唾液样本分类良好,灵敏度及特异性较好;进一步验证了AFP抗原的分子组成,且AFP抗原的拉曼强度基本随浓度的递增呈递减趋势;对肝癌患者的唾液样本及AFP抗原的拉曼光谱进行比对分析,提示利用表面增强拉曼光谱检测唾液有可能诊断早期癌症。
Surface-enhanced Raman spectroscopy is a rapid detection technique of trace-level marker in recent years. It has reached the level of molecular recognition, has high sensitivity, and can accurately analyze and understand the structure and composition of matter. Human saliva contains a large number of signs related to specific proteins and secretions. Saliva testing with non-invasive, easy access, suitable for large-scale census and so on. Surface-enhanced Raman spectroscopy can rapidly detect saliva, and the combination of the two may become a new method of cancer diagnosis. In this paper, surface-enhanced spectroscopy was used to study the patients with liver cancer. The detection of saliva, the treatment of samples, the collection and analysis of spectra, the detection and analysis of liver cancer-specific alpha fetoprotein (AFP) Relevant characteristic information showed that the salivary samples of patients with hepatocellular carcinoma and normal had good classification and good sensitivity and specificity. The molecular composition of AFP antigen was further verified, and the Raman intensity of AFP antigen showed a decreasing trend with increasing concentration. Saliva samples from patients and AFP antigen Raman spectra were compared, suggesting that the use of surface enhanced Raman spectroscopy saliva may be diagnosed early cancer.