猫爪草已经临床治疗耐药结核病,但其作用机理和有效成分尚不清楚。为研究其可能的作用靶标,采用双向电泳技术比较分析猫爪草提取物作用前后结核分枝杆菌临床分离株的全细胞蛋白表达差异。发现22个蛋白质斑点具有明显差异,对其中3个表达明显下调的蛋白质斑点进行基质辅助激光解吸电离飞行时间质谱分析,获得了肽质量指纹图谱。数据库检索分析确定这3个点代表的蛋白质分别为硫代硫酸硫转移酶,延长因子Ts和热休克蛋白X,分别参与厌氧硫代谢、蛋白质翻译和蛋白质折叠分泌、转录调控等过程。这有助于深入研究猫爪草对结核分枝杆菌的作用机理,也为发现新的抗结核病治疗药物靶标提供了线索。 Cat’s claw grass has been used to treat drug-resistant tuberculosis clinically, but its mechanism of action and effective ingredients are still unclear. In order to study its potential targets, the two-dimensional electrophoresis technique was used to compare and analyze the differences in the expression of the whole cell proteins of clinical isolates of Mycobacterium tuberculosis before and after the action of the extract of Caterpillar. 22 protein spots were found to be significantly different. Three of the protein spots that were significantly down-regulated were analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry to obtain peptide mass fingerprints. The database search analysis confirmed that the proteins represented by these three points were thiosulfuric acid sulfur transferase, elongation factor Ts and heat shock protein X, which were involved in anaerobic sulfur metabolism, protein translation, protein folding and secretion, and transcription regulation. This will provide insight into the mechanism of action of cat’s claw on Mycobacterium tuberculosis and provide clues for the discovery of new drug targets for anti-tuberculosis treatment.