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胚发育指数分别为0.15和0.40的‘西农早蜜’桃幼胚在胚珠离体培养条件下维持胚性生长,转移胚培养后各获得77.8％和100％的成苗率,克服了桃幼胚营养中存在的早熟萌发、成苗率低等问题。此外,取盛花后10d(胚处于二细胞原胚期)、17d(球形胚)、24d(球形胚)、31d(心形胚)、38d和45d(肉眼看不到的幼胚)的胚珠进行培养,分别获得1.7％,3.4％,14.3％,22.4％,53.4％和57.9％的成苗率,为克服李属果树种间杂交桃胚早期败育的障碍,提供了新方法。本文还研究了影响胚珠培养的有关因素。
Embryo development index of 0.15 and 0.40 of ’Xinong early honey’ peach embryos in embryo culture in vitro maintained embryogenic growth conditions, the transfer embryo culture obtained 77.8% and 100% of the seedling rate, to overcome the peach young Premature germination of embryo nutrition, seedling rate is low and other issues. In addition, the ovules at 10 days after flowering (embryos at the stage of two-cell embryo), 17 days (globular embryos), 24 days (globular embryos), 31 days (embryoid heart), 38 days and 45 days And achieved the seedling rates of 1.7%, 3.4%, 14.3%, 22.4%, 53.4% and 57.9%, respectively, which provided a new method for overcoming the obstacles of early abortion of interspecific hybrid peach embryos in Prunus armeniaca. This article also studied the impact of ovule culture related factors.