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目的研究模拟试验中回收草莓上金黄色葡萄球菌的最佳方法,为以后的风险评估提供基础研究依据。方法对草莓进行不同类型的污染处理,于25℃条件下贮存,在不同时间点用不同样品处理方式进行金黄色葡萄球菌回收并计数。结果 0.1%蛋白胨水和去离子水作为菌体载体对金黄色葡萄球菌在草莓上的生长影响差异有统计学意义(P<0.05),用0.1%蛋白胨水作为菌体载体能更好的模拟真实污染情况并得到更好的回收效果。均质和振荡处理对金黄色葡萄球菌回收效果影响差异无统计学意义(P>0.05),不同来源金黄色葡萄球菌的生长情况差异无统计学意义(P>0.05)。25℃贮存条件下,草莓上金黄色葡萄球菌在36 h内成倍增长。结论金黄色葡萄球菌在草莓上生长模拟试验发现,载体的选择非常重要。在其他风险评估模拟试验中,也应考虑实际污染的各方面因素来设计方案才能得到最真实的试验结果,为微生物风险评估提供科学依据。
Objective To study the best method of recovering Staphylococcus aureus from strawberries in the simulation experiment and provide the basis for the future risk assessment. Methods Strawberry was treated with different types of pollution and stored at 25 ℃. Staphylococcus aureus was collected and counted at different time points by different sample treatments. Results The effect of 0.1% peptone water and deionized water as carrier on the growth of S. aureus on strawberry was statistically significant (P <0.05). Using 0.1% peptone water as carrier of cell could better simulate the real Pollution and get better recovery results. The effect of homogenization and shaking treatment on the recovery of Staphylococcus aureus was not statistically different (P> 0.05). There was no significant difference in the growth of Staphylococcus aureus between different sources (P> 0.05). Staphylococcus aureus in strawberry increased exponentially within 36 h at 25 ℃. Conclusion Staphylococcus aureus in strawberry growth simulation test found that the choice of vector is very important. In other risk assessment simulation experiments, the most realistic experimental results should be designed considering all aspects of actual pollution, so as to provide a scientific basis for microbial risk assessment.