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本文小结了治癌中药的选择及治癌中药药效的筛选判断结果。实验方法〔检品的调制〕①水浸膏:药材按需要粉粹,加10倍量纯水,于水浴上加热1小时,共提取两次,合并提取液,滤过,冷冻干燥。②甲醇浸膏:药材适当粉粹,加10倍量甲醇回流1小时,抽提两次,合并提取液,减压蒸去溶媒,在干燥器中充分干燥。〔抗癌筛选〕①体内试验:用接种欧立希(Ehrlich)腹水癌的小鼠,以改良的总细胞容积法判断结果。操作如下:将欧氏腹水癌细胞移植到约重20克的 ddy 种雄性小鼠腹腔内(10~7个细胞/鼠),试验药物溶解或混悬于生理盐水中,于移植后第五日起
This article summarizes the selection of cancer drugs and screening results of cancer drug efficacy. Experimental method [Preparation of the test product] 1 Water extract: The medicinal material is powdered as required, plus 10 times pure water, heated on a water bath for 1 hour, and extracted twice in total. The extracts are combined, filtered, and freeze-dried. 2 Methanol Extract: The medicinal material is properly powdered, plus 10 times the amount of methanol refluxed for 1 hour, extracted twice, and the combined extracts were evaporated under reduced pressure to remove the solvent and dried in a desiccator. [Anticancer screening] 1 In vivo test: The mice were vaccinated with Ehrlich ascites carcinoma and the results were judged by the improved total cell volume method. The procedure was as follows: Ehrlich ascites cancer cells were transplanted into the abdominal cavity (10~7 cells/rat) of about 20 g of ddy male mice, and the test drugs were dissolved or suspended in physiological saline on the fifth day after transplantation. From