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目的：从血浆中分离获得低密度脂蛋白（ＬＤＬ）和进行９９ｍ锝（９９ｍＴｃ）标记，为ＬＤＬ作为抗癌靶向载体研究作准备。方法：用超速离心法从血浆中分离ＬＤＬ，从提取分离效果得到较好的梯度分离条件。采用连亚硫酸钠还原法对ＬＤＬ进行９９ｍＴｃ标记。结果：密度１．００６，１．０１９，１．０６３的ＫＢｒ液和１．２１０的血浆ＫＢｒ密度液，以１．５∶２．０∶３．０∶５．０组成不连续密度梯度，经４℃、４００００ｒ·ｍｉｎ－１、２４ｈ超速离心，可一步分离获得电泳纯的ＬＤＬ。采用连亚硫酸钠还原高锝酸进行放射性锝标记，标记率可达３９．７３％。结论：密度梯度法可一次超速离心提取获得纯度较高的ＬＤＬ，并可用９９ｍＴｃ标记后用于进一步研究。
OBJECTIVE: To isolate low density lipoprotein (LDL) and carry out 99mT (99mTc) labeling from plasma to prepare LDL as anticancer targeting carrier. Methods: LDL was separated from plasma by ultracentrifugation, and a good gradient separation condition was obtained from the extraction and separation. LDL was labeled with 99mTc using sodium sulfite reduction. RESULTS: Densities of 1.006, 1.019, and 1.063 KBr fluids and 1.210 plasma KBr density fluids consisted of discontinuous density gradients of 1.5:2.0:3.0:5.0. The electrophoretically pure LDL can be isolated in one step by ultracentrifugation at 4°C, 40000r·min-1, 24h. Using sodium sulfite to reduce the pertechnic acid for radioactive hydrazine labeling, the labeling rate can reach 39.73%. Conclusion: The density gradient method can be extracted by one-time ultracentrifugation to obtain high-purity LDL, which can be labeled with 99mTc for further study.