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目的 分析糖尿病长爪沙鼠5种组织中ND3基因在mRNA和蛋白水平的表达,以期探索长爪沙鼠糖尿病模型中ND3的作用及其靶器官.方法 选取血糖正常对照组沙鼠和糖尿病实验组沙鼠各6只,分别采集动物的肾脏、肝脏、骨骼肌、脑和心脏组织,采用实时定量PCR和Western blotting方法检测在各组织中ND3 mRNA和ND3蛋白水平表达状况.结果 与对照组动物相比,糖尿病长爪沙鼠ND3 mRNA表达水平在肾脏组织中极显著升高,肝脏和肌肉组织中则显著降低,脑组织中仅有降低趋势,而在心脏组织中仅有升高趋势.检测ND3蛋白水平表明,除心脏组织外,其他组织均与mRNA水平表达结果一致.结论 肾脏组织可能是糖尿病长爪沙鼠ND3基因作用的主要部位,同时在其他4种组织中该基因也发挥着一定作用.“,”Objective To investigate the mRNA and protein expression level of NADH dehydrogenase 3(ND3) gene in 5 different tissues of diabetic gerbils,to understand the location and target tissue of ND3 in diabetic gerbil model.Methods Six gerbils for control and diabetic groups were selected respectively.The kidney,liver,skeletal muscle,brain and heart from each group were collected.The mRNA and protein expression of ND3 were evaluated by Real time-PCR and Western blotting in both groups.Results The mRNA expression of ND3 in diabetic group was extremely significantly increased in kidney,and significantly decreased in liver and skeletal muscle compared with those of control animal.While it just showed decreasing tendency in brain and increasing tendency in heart tissue.The detection of protein expression showed that the protein level was consistent with mRNA expression in all tissues except heart.Conclusions The kidney may be the target tissue of ND3 in diabetic gerbil.In addition,ND3 also exhibits effect in other 4 tissues of diabetic gerbil.