目的　观察恶性肿瘤患儿外周血T淋巴细胞嗜银核仁形成区 (Ag NORs)酸性非组蛋白表达活性的改变及其与T淋巴细胞亚群的关系。方法　对肿瘤组 30例 ,正常组 30例应用细胞培养及普通细胞银染技术进行外周血淋巴细胞染色 ,通过KL型肿瘤免疫图像分析系统测定Ag NORs银染面积与核面积的比值 (IS % ) ;应用流式细胞仪测定T淋巴细胞表面抗原。结果　肿瘤组患儿的CD4及CD4 /CD8降低 ,CD8升高 ,与正常组比较具有显著性差异 (t =3 85 ,4 6 0 ,- 2 4 5 ,P均 <0 0 5 ) ,淋巴细胞IS %均值低于正常组 (t =7 14 ,P <0 0 1) ,其变化与CD3、CD4、CD8、CD4 /CD8比较无明显相关性 (相关系数r分别为 - 0 16 37,- 0 172 6 ,0 32 4 5 ,- 0 32 19,均为P >0 0 5 )。结论　恶性肿瘤患儿T淋巴细胞嗜银核仁形成区酸性非组蛋白表达活性下降 ,T细胞免疫功能低下 Objective To investigate the changes of acidic non-histone protein expression in peripheral blood mononuclear cells (T-lymphocytes) and their relationship with T-lymphocyte subsets in children with malignant tumor. Methods Peripheral blood lymphocytes were stained with cell culture and ordinary cell silver staining in 30 cases of tumor group and 30 cases of normal group. The ratio of silver staining area to nucleus area (Ag%) was determined by KL tumor immunoassay system. ; T lymphocyte surface antigen was determined by flow cytometry. Results The CD4 and CD4 / CD8 and CD8 in the tumor group were significantly lower than those in the normal group (t = 3 85, 4 6 0, - 2 45, P 0 05), lymphocytes The mean IS% was lower than that of the normal group (t = 7 14, P <0.01), and the change was not significantly correlated with the levels of CD3, CD4, CD8 and CD4 / CD8 (r = -0.1637, -0 172 6, 0 32 4 5, - 0 32 19, all P> 0 0 5). Conclusion The expression of acidic non-histone protein in T-lymphocyte of silver-forming nuclei of T-lymphocytes in children with malignant tumor is decreased, while the immune function of T-cell is low