论文部分内容阅读
为探讨胸腺在重症肌无力发病机制中的作用 ,取重症肌无力患者手术摘除胸腺制备胸腺提取液 ,进行聚丙烯酰胺凝胶电泳 ,并利用细胞培养和细胞增殖试验观察胸腺提取液对正常人外周血单个核细胞增殖的影响 ,同时利用酶联免疫吸附试验测定其血清AchRab的含量。经聚丙烯酰胺凝胶电泳分析发现约 34 %左右的重症肌无力患者胸腺提取液中存在分子量约为 110 0 0的蛋白 ,这些患者的胸腺提取液对外周血淋巴细胞增殖有显著的抑制作用 ,在胸腺摘除后血清AchRab水平也有更显著的下降 ,与胸腺提取液中没有这种蛋白的重症肌无力患者相比有显著差异 (P <0 0 1)。说明部分重症肌无力患者胸腺提取液蛋白成分和生物学活性异常 ,并可能与自身抗体的产生有关。
To explore the role of the thymus in the pathogenesis of myasthenia gravis, thymus extract was prepared by surgical removal of the thymus from patients with myasthenia gravis. Polyacrylamide gel electrophoresis was performed on the thymus, and the effect of thymus extract on the peripheral Blood mononuclear cells proliferation, while the use of enzyme-linked immunosorbent assay serum AchRab content. Polyacrylamide gel electrophoresis analysis found that about 34% of myasthenia gravis patients with thymus extract molecular weight of about 110 0 protein, thymus extract of these patients on peripheral blood lymphocyte proliferation was significantly inhibited, Serum AchRab also showed a more significant decrease after thymus ablation, which was significantly different from that of patients with myasthenia gravis without thymus extract (P <0.01). Some patients with myasthenogenic thymus extract protein composition and biological activity abnormalities, and may be related to the production of autoantibodies.