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目的:测定“三热论”方各纯化部位中药根碱、表小檗碱、黄连碱、巴马汀和小檗碱的含量。方法:“三热论”复方经HPD 400大孔树脂,分别用10%,30%,60%,90%的乙醇洗脱后,采用HPLC测定4个洗脱部位中5种生物碱的含量。结果:HPD 400大孔树脂纯化后,30%乙醇洗脱部位生物碱含量最高。结论:大孔树脂纯化法简单易行,30%纯化部位经HPLC检测,其中生物碱含量最高,为有效部位筛选奠定了基础。
Objective: To determine the content of traditional Chinese medicine, epinephrine, berberine, berberine, palmatine and berberine in the purified fractions of “three heat theory”. Methods: After the compound HPD 400 macroporous resin was eluted with 10%, 30%, 60% and 90% ethanol respectively, the five alkaloids in the four eluted sites were determined by HPLC content. Results: After the HPD 400 macroporous resin was purified, the alkaloid content was highest in the elution fraction of 30% ethanol. Conclusion: The macroporous resin purification method is simple and easy. The 30% purification site is detected by HPLC, and the alkaloid content is the highest, which lays the foundation for the effective site screening.