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Objective:To investigate the effects of triptolide(TPL)on cell growth,cell cycle and the expressions of p21wapl/cip1 and p27kip1. Methods:MTT assay was used to determine the cell viability after triptolide treatment in human multiple myeloma RPMI-8226 cells.The effect on cell cycle distribution was determined by flow cytometry. Semi-quantitative reverse transcription-PCR was used to examine the mRNA expressions of p21wap1/cip1 and p27kip1.The protein expressions of p21 wap1/cip1 and p27kip1 were determined by Western blot. Results:Triptolide of varying concentrations induced cell viability inhibition in dose-and time-related fashion and caused G_0-G_1 phase arrest of cell cycle progression in RPMI-8226 cells.These effects accompanied with up-modulation of the expressions of p21 wap1/cip1 and p27kip1. Conclusion:These results suggest that triptolide inhibit cell proliferation and cell cycle progression via up-regulating p21wap1/cip1 and p27kip1 and triptolide may exert its anti-cancer activity through this pathway.
Objective: To investigate the effects of triptolide (TPL) on cell growth, cell cycle and the expressions of p21wapl / cip1 and p27kip1. Methods: MTT assay was used to determine the cell viability after triptolide treatment in human multiple myeloma RPMI- 8226 cells. The effect on cell cycle distribution was determined by flow cytometry. Semi-quantitative reverse transcription-PCR was used to examine the mRNA expressions of p21wap1 / cip1 and p27kip1. The protein expressions of p21 wap1 / cip1 and p27kip1 were determined by Western blot. : Triptolide of varying concentrations of cell viability inhibition in dose-and time-related fashion and caused G_0-G_1 phase arrest of cell cycle progression in RPMI-8226 cells. These effects accompanied with up-modulation of the expressions of p21 wap1 / cip1 and p27kip1. Conclusion: These results suggest that triptolide inhibit cell proliferation and cell cycle progression via up-regulating p21wap1 / cip1 and p27kip1 and triptolide may exert its anti-cancer a ctivity through this pathway