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目的从血管生成的角度探讨益气养阴活血法及其代表方芪棱汤抗大鼠脑缺血再灌注损伤的机理。方法将大鼠随机分为假手术组、模型组、芪棱汤去养阴药组(对照组)、芪棱汤组(治疗组)。采用颈内动脉线栓法复制大鼠脑缺血再灌注(MCAO/Reperfusion)模型。在再灌注不同时点采用免疫组织化学法测血管内皮生长因子(VEGF)、血管紧张素(Ang)-2,CD34标记微血管计数。结果假手术组VEGF表达、Ang-2表达极弱;除缺血再灌注6h外,各相应再灌注时间点12h、1d、3d、7d,治疗组及对照组VEGF表达、Ang-2表达较模型组明显增多,且治疗组与对照组比较有显著差异。CD34标记微血管计数再灌注各时间点,治疗组、对照组与模型组比较均有显著差异;治疗组与对照组比较有显著差异。结论芪棱汤通过促进VEGF、Ang-2表达的上调,促进微血管新生,可能是其抗脑缺血再灌注损伤的作用机制之一;益气养阴活血法优于益气活血法。
Objective To investigate the mechanism of Yiqi Yangyinhuoxue and its representative Fangqianling decoction on cerebral ischemia-reperfusion injury in rats from the perspective of angiogenesis. Methods The rats were randomly divided into sham-operation group, model group, Qi-Ling-Tang nourishing yin-yin group (control group) and Qileng Decoction group (treatment group). The rat model of cerebral ischemia-reperfusion (MCAO / Reperfusion) was reproduced by the method of internal carotid artery suture. At different time points after reperfusion, the vascular endothelial growth factor (VEGF), angiotensin (Ang) -2 and CD34-labeled microvessel counts were detected by immunohistochemistry. Results The expressions of VEGF and Ang-2 in sham-operated group were very weak. The reperfusion time of each group was 12h, 1d, 3d and 7d except for 6h after reperfusion. Compared with model Group was significantly increased, and the treatment group and the control group were significantly different. There were significant differences between the treatment group, the control group and the model group at each time point of CD34-labeled microvascular reperfusion; there was a significant difference between the treatment group and the control group. Conclusion Qileng Decoction can promote angiogenesis by upregulating the expression of VEGF and Ang-2, which may be one of the mechanisms of its anti-cerebral ischemia-reperfusion injury.