目的：为医学研究长期提供培养的人胚细胞。方法：体外培养人胚细胞，观察其生长特性、染色体数目以及冻存和复苏的条件。结果：培养的人胚肺、皮肤、肾、脾及脑组织中，肺和皮肤细胞在冻存前后增长速度较快而稳定，染色体数目为２ｎ＝４６，占８７％～９１％；肾、脾细胞扩增缓慢；脑组织几乎不能扩增。结论：人胚肺和皮肤细胞可长期培养，可为医学研究提供二倍体细胞实验对象。 Objective: To provide cultured human embryonic cells for medical research for a long time. Methods: Human embryonic cells were cultured in vitro and their growth characteristics, number of chromosomes and the conditions of cryopreservation and resuscitation were observed. Results: The lung and skin cells grew rapidly and steadily in human embryo lung, skin, kidney, spleen and brain tissue before and after cryopreservation. The number of chromosomes was 2n = 46, accounting for 87% ~ 91%. Slow cell proliferation; almost impossible to amplify brain tissue. Conclusion: Human embryo lung and skin cells can be cultured for a long time, which can provide diploid cells for medical research.