为进一步探讨氯喹对油酸所致的实验性大鼠呼吸窘迫综合征(RDS)的治疗作用机理,我们实验观察了用药前后大鼠肺中β-即受体的变化以及由此引起的cAMP含量的变化. 研究中用放射性配基结合分析法测定β-受体的最大结合容量(Bmax)和解离常数(Kd),以竞争性结合蛋白分析法测定cAMP含量。实验结果显示:正常大鼠组、RDS模型组和氯喹治疗组大鼠肺中的Bmax值分别为490±61、307±55、494±48fmol/mg蛋白;各组Kd值无明显改变;其相应各组大鼠肺中cAMP To further explore the mechanism of action of chloroquine on oleosinic-induced experimental rat respiratory distress syndrome (RDS), we observed the changes of beta -receptors in the lungs of rats before and after treatment and the cAMP content The maximal binding capacity (Bmax) and dissociation constant (Kd) of β-receptor were determined by radioligand binding assay, and the cAMP content was determined by competitive binding protein assay. The results showed that the Bmax values ​​of the normal rats, RDS model group and chloroquine treatment group were 490 ± 61,307 ± 55,494 ± 48fmol / mg protein, respectively; the Kd values ​​of each group did not change significantly; The cAMP in lung of each group