目的:考察了海藻多糖多次给药(14~17 d)对小鼠抗微生物能力的影响。方法:取雄性昆明种小鼠,按体质量随机分为8组,分别为空白对照组、正常对照组、模型组、紫芝多糖1 000 mg/kg、海藻多糖125、250、500和1 000 mg/kg剂量组,每组12只动物,每天早8:00禁食,下午2:00根据其体质量灌胃给药,用量体积为0.4 mL/20 g,空白组不给药和溶媒,正常和模型组给予同体积的0.5%的CMC液,给药2 h后按3 g/只动物的量给食,共给药15 d,于给药第6天除空白和正常对照组外其余所有小鼠皮下一次注射环磷酰胺100 mg/kg造模,末次给药24 h后小鼠眼眶取血,分离血清测定其抑菌圈,试验结果用采用统计学处理。结果:用海藻多糖125、250、500和1 000 mg/kg经口灌胃后,能明显增大环磷酰胺所致免疫低下和荷瘤S180瘤小鼠血清的抑菌能力,用海藻多糖250、500和1 000 mg/kg经口灌胃后,能明显增加经放射损伤小鼠血清的抑菌能力;用海藻多糖500 mg/kg灌胃小鼠感染绿脓假单胞菌后72 h内死亡率明显下降;海藻多糖对金黄色葡萄球菌感染小鼠有明显的保护作用;用海藻多糖500 mg/kg剂量能明显减少小鼠感染流感病毒后的死亡率。结论:由此可见,海藻多糖可以通过提高免疫而产生一定的抵抗细菌和病毒感染的能力。 OBJECTIVE: To investigate the effect of polysaccharide from seaweed on antimicrobial abilities in mice administered multiple times (14-17 days). METHODS: Male Kunming mice were randomly divided into 8 groups according to their body weight, which were blank control group, normal control group, model group, 1 000 mg / kg polysaccharides, 125, 250, 500 and 1 000 mg / kg dose group, each group of 12 animals, fasting at 8:00 am every day, 2:00 according to their body weight intragastric administration, the volume of 0.4 mL / 20 g, the blank group was not administered and vehicle, normal And the model group were given the same volume of 0.5% CMC solution, administered 2 g after 3 g / animal feed for a total of 15 d, on the 6th day except the blank and the normal control group all the rest The mice were injected subcutaneously with cyclophosphamide at 100 mg / kg for 24 h after the last administration, orbital blood was collected from the mice and the antibacterial circle was separated from the serum. The test results were statistically analyzed. Results: After oral administration of 125, 250, 500 and 1000 mg / kg seaweed polysaccharides, the immunosuppressive effect of cyclophosphamide and the antimicrobial activity of S180 tumor-bearing mice serum were obviously increased. , 500 and 1000 mg / kg orally after oral administration, can significantly increase the antimicrobial activity of serum-irradiated mice; with seaweed polysaccharide 500 mg / kg intragastric administration of mice infected with Pseudomonas aeruginosa within 72 h The death rate decreased significantly; seaweed polysaccharide has a significant protective effect on mice infected with Staphylococcus aureus; with seaweed polysaccharide 500 mg / kg dose can significantly reduce the mortality of mice infected with influenza virus. CONCLUSION: It can be seen that seaweed polysaccharides can produce some resistance to bacterial and viral infections by enhancing immunity.