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目的研究二氢丹参酮(DHT)对人胃癌SGC7901细胞增殖、迁移和侵袭能力的影响,并探讨其作用的分子机制。方法利用不同浓度的DHT处理细胞后,MTT法检测DHT对细胞生长活力的影响,划痕实验观察DHT对细胞运动能力的影响,Transwell小室模型研究DHT对细胞迁移和侵袭的影响,q RT-PCR和Western blotting检测基质金属蛋白酶MMP2、MMP9和Hedgehog通路调控基因Gli1和HHIP的m RNA和蛋白表达水平。结果与对照组相比,DHT可显著抑制SGC7901细胞的体外增殖及迁移能力,Transwell实验表明药物处理后穿膜细胞数明显低于对照组,并且呈剂量依赖性。Western blotting和q RT-PCR实验结果表明,DHT可显著抑制SGC7901细胞中MMP9的表达,降低Gli1基因的m RNA和蛋白表达,并提高HHIP基因的表达水平。结论 DHT能够抑制SGC7901细胞的迁移和侵袭能力,其机制可能与MMP9蛋白的表达降低和Hedgehog通路活性抑制有关。
Objective To investigate the effects of DHT on the proliferation, migration and invasion of human gastric cancer SGC7901 cells and to explore its molecular mechanism. Methods The cells were treated with different concentrations of DHT. The effects of DHT on cell viability were detected by MTT assay. The effects of DHT on cell motility were observed by scratch assay. The effects of DHT on cell migration and invasion were investigated by Transwell chamber model. Western blotting was used to detect the mRNA and protein levels of MMP2, MMP9 and Hedgehog pathway regulatory genes Gli1 and HHIP. Results Compared with the control group, DHT significantly inhibited the proliferation and migration of SGC7901 cells in vitro. Transwell assay showed that the number of transmembrane cells was significantly lower than that of the control group in a dose-dependent manner. The results of Western blotting and q RT-PCR showed that DHT could significantly inhibit the expression of MMP9, decrease the mRNA and protein expression of Gli1 and increase the expression of HHIP in SGC7901 cells. Conclusions DHT can inhibit the migration and invasion of SGC7901 cells. The mechanism may be related to the decreased expression of MMP9 protein and the inhibition of Hedgehog pathway activity.